Original Articles ROLE OF PROGNOSTIC VARIABLES OF MEDICAL IMPORTANCE AND THEIR INTERPLAY IN LEUKEMIA: A STUDY FROM LOCAL POPULATION

Abstract

Introduction:

Leukemia is defined as the cancer of blood-forming tissues. It is equally common in children and adults. It involves abnormal production of white blood cells (WBCs) which are primarily responsible for the defense in the human body thus, abnormality in the production of WBCs leads to the failure in combating the infection. Aim of the current study is to rule out the significant markers of prognostic importance that play an important role in the development of leukemia in the local population

Material and Methods:

Thirty (n=30) patients of leukemia and thirty (n=30) healthy controls were enrolled for the current study by random sampling. This cross sectional study was approved by the Departmental Research Committee (DRC), Institute of Molecular Biology and Biotechnology (IMBB), the University of Lahore. Blood and Saliva samples were collected and subjected for the analysis of the MDA, isoprostanes, Interleukin, MPO, and Neutrophils levels with the help of their respective protocols.

Results:

Results of this study showed that the levels of oxidative stress markers and interleukins were significantly increased in patients with leukemic disorders as compared with the healthy subjects. It showed that levels of MDA, isoprostanes, 8-OHdG, TNF-α and interleukin-6 were significantly higher (p-value = 0.019, 0.001, 0.041, 0.008 and 0.016 respectively) in the serum and saliva samples of patients as compared to that in the healthy subjects. Levels of MPO and Neutrophils presented significantly (p-value= 0.043, 0.007) higher levels in the blood samples whereas, these were not detected in the saliva samples of the patients.

Conclusion:

The current study suggests the significant role of oxidative stress markers in the initiation and progression of leukemia. It shows levels of interleukin and markers of DNA damage remained elevated in the patients with leukemia as compared to that of healthy individuals. Therefore, therapy with significant antioxidants can improve the status of individuals suffering from leukemia in the local population.